3-D Life PVA-CD Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are polymeric SG-PVA and CD-Link crosslinker. When combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, resulting in gel formation. Slow gelation kinetics allows time to manipulate the solution before onset of gel formation. CD-Link is composed of PEG and an MMP-cleavable peptide designed for a broad range of MMP cleavage including MMP-1, 3, 7 and 9. It allows cells to spread and migrate within the gel if they express the indicated MMPs. Cell spreading and migration usually requires the presence of adhesion peptides. Prior to the cross-linking step, peptides can be attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix. QUANTITY: Allows formation of up to 2 ml 3-D Life Hydrogel depending on gel stiffness.